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Addgene

p203 Dync1li1 N235Y
(Plasmid #26451)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC-A-amp/kan
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    subcloning and sequencing

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    SoloPack [Stratagene], standard LB with 50 ug/ml of kanamicin
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytoplasmic dynein light intermediate chain 1 N235Y
  • Alt name
    Dync1li1, DLIC
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1595
  • Mutation
    changed Asparagine 235 to Tyrosine
  • GenBank ID
    NM_146229
  • Entrez Gene
    Dync1li1 (a.k.a. 1110053F02Rik, Dlic1, Dnclic1, LIC-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p203 Dync1li1 N235Y was a gift from Elizabeth Fisher (Addgene plasmid # 26451 ; http://n2t.net/addgene:26451 ; RRID:Addgene_26451)
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