p196 Dync1i2.A (Ex 1a)
(Plasmid
#26446)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3931
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Vector typesubcloning and sequencing
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10 [Invitrogen], standard LB with 50 ug/ml of kanamicin
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1a)
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Alt nameDync1i2, IC74, DIC
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SpeciesM. musculus (mouse)
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Insert Size (bp)2442
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MutationN/A
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GenBank IDNM_010064
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Entrez GeneDync1i2 (a.k.a. 3110079H08Rik, AW554389, Dnci, Dncic2)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13, T7
- 3′ sequencing primer M13, T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p196 Dync1i2.A (Ex 1a) was a gift from Elizabeth Fisher (Addgene plasmid # 26446 ; http://n2t.net/addgene:26446 ; RRID:Addgene_26446) -
For your References section:
Mouse cytoplasmic dynein intermediate chains: identification of new isoforms, alternative splicing and tissue distribution of transcripts. Kuta A, Deng W, Morsi El-Kadi A, Banks GT, Hafezparast M, Pfister KK, Fisher EM. PLoS One. 2010 . 5(7):e11682. 10.1371/journal.pone.0011682 PubMed 20657784