p164 Dync1i1.B
(Plasmid #26435)

• Depositing Lab: Elizabeth Fisher
• Publication: Kuta et al PLoS One. 2010 . 5(7):e11682.

Backbone

• Vector backbone: pCR4-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3956
• Vector type: subcloning and sequencing

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10 [Invitrogen], standard LB with 50 ug/ml of kanamicin
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cytoplasmic dynein 1 intermediate chain 1 isoform B
• Alt name: Dync1i1, IC74, DIC
• Species: M. musculus (mouse)
• Insert Size (bp): 2490
• Mutation: N/A
• GenBank ID: NM_010063
• Entrez Gene: Dync1i1 (a.k.a. DH IC-1, DHIC-1, DI, DIC, Dnci, Dncic1, IC7, IC74)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13, T7
• 3′ sequencing primer: M13, T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry