pLenti CMVtight Hygro DEST (w769-1)
(Plasmid #26433)

• Purpose: (Empty Backbone)
• Depositing Lab: Eric Campeau
• Publication: Tet-On Advanced vectors (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size (bp): 10104
• Vector type: Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: DB3.1 or ccDB survival, 37oC
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: no insert

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVtightFORW
• 3′ sequencing primer: WPRErev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: The CMVtight element was a gift from Dr. Dominic Esposito.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.

Addgene found this plasmid to be extremely slow growing.