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Addgene

pLenti CMVtight Puro DEST (w768-1)
(Plasmid #26430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size (bp) 9402
  • Vector type
    Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1 or ccDB survival, 37oC.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    no insert

Cloning Information

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The CMVtight element was a gift from Dr. Dominic Esposito.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMVtight Puro DEST (w768-1) was a gift from Eric Campeau (Addgene plasmid # 26430 ; http://n2t.net/addgene:26430 ; RRID:Addgene_26430)
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