pLenti CMV rtTA3 Blast (w756-1)
(Plasmid #26429)

• Purpose: 3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast
• Depositing Lab: Eric Campeau
• Publication: Tet-On Advanced vectors (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 7681
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetracycline repressor A3 mutant
• Alt name: rtTA3
• Insert Size (bp): 707

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVforw
• 3′ sequencing primer: WPRErev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The rtTA3 element was a gift from Dr. Dominic Esposito.
• Articles Citing this Plasmid:
  • 47 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use to make them Tet-On Advanced.