pTitin
(Plasmid #26428)

• Depositing Lab: Didier Trono
• Publication: Tono lab plasmid (unpublished)

Backbone

• Vector backbone: pRRL.SIN.cPPT.PGK.GFP
• Backbone size w/o insert (bp): 7400
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: The Trono lab typically amplifies this plasmid in the HB101 strain but it will be provided in Stbl3 from Addgene, as it is also a rec- strain.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: partial sequence of mouse Titin
• Species: M. musculus (mouse)
• Insert Size (bp): 121

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry