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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5'dGFP3'
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Alt nameGFP reporter
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Insert Size (bp)4100
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Tags
/ Fusion Proteins
- dGFP
- CamKIIa 5'-UTR
- CamKIIa 3'-UTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PmeI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see the supplemental documents for extensive information about how this plasmid was made.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1-5'dGFP3' was a gift from Erin Schuman (Addgene plasmid # 26425 ; http://n2t.net/addgene:26425 ; RRID:Addgene_26425) -
For your References section:
Regulation of dendritic protein synthesis by miniature synaptic events. Sutton MA, Wall NR, Aakalu GN, Schuman EM. Science. 2004 Jun 25. 304(5679):1979-83. 10.1126/science.1096202 PubMed 15218151