pBS XPLCg1
(Plasmid
#26424)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26424 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephospholipase C-gamma-1
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Alt namePLC-gamma-1
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SpeciesX. laevis (frog)
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Insert Size (bp)4819
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GenBank IDAB287408
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Entrez GeneLOC398360 (a.k.a. XPLCG1a)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS XPLCg1 was a gift from Masahiro Yamagishi (Addgene plasmid # 26424 ; http://n2t.net/addgene:26424 ; RRID:Addgene_26424) -
For your References section:
Competition for ligands between FGFR1 and FGFR4 regulates Xenopus neural development. Yamagishi M, Okamoto H. Int J Dev Biol. 2010 . 54(1):93-104. 10.1387/ijdb.092849my PubMed 20013652
Map uploaded by the depositor.