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Addgene

pBS XPLCg1
(Plasmid #26424)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26424 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phospholipase C-gamma-1
  • Alt name
    PLC-gamma-1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    4819
  • GenBank ID
    AB287408
  • Entrez Gene
    LOC398360 (a.k.a. XPLCG1a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS XPLCg1 was a gift from Masahiro Yamagishi (Addgene plasmid # 26424 ; http://n2t.net/addgene:26424 ; RRID:Addgene_26424)
  • For your References section:

    Competition for ligands between FGFR1 and FGFR4 regulates Xenopus neural development. Yamagishi M, Okamoto H. Int J Dev Biol. 2010 . 54(1):93-104. 10.1387/ijdb.092849my PubMed 20013652