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Addgene

pHDAdVenus-geo-TK Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: BamHI and HindIII (separate digests)

We expected the following band sizes for these digests:
BamHI -- 2.8kb, 3.0kb, 4.8kb, 5.6kb, adn 15.2kb
HindIII -- 167bp (run off gel), 1.0kb, 4.0kb, 5.2kb(X2), 6.5kb, and 9.3kb

We see the expected pattern.