pHDAdVenus-geo-TK
(Plasmid #26423)

• Depositing Lab: Kohnosuke Mitani
• Publication: Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13781-6.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 2880
• Vector type: Mammalian Expression, Adenoviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGK-b-geo, HSV-TK, CAG-Venus
• Species: E. coli, HSV, jellyfish
• Insert Size (bp): 28690

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI, SwaI, SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that Addgene was unable to obtain very much sequence for this plasmid, but our diagnostic digest (see Reviews) suggests that it is correct. We encourage users to do their own diagnostics.