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Addgene

pBRHDAd-TKVenus
(Plasmid #26422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 3380
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HSV-TK, CS2-Venus
  • Species
    jellyfish
  • Insert Size (bp)
    5180

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI, SalI, Sse8387I (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBRHDAd-TKVenus was a gift from Kohnosuke Mitani (Addgene plasmid # 26422 ; http://n2t.net/addgene:26422 ; RRID:Addgene_26422)

  • For your References section:

    Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors. Suzuki K, Mitsui K, Aizawa E, Hasegawa K, Kawase E, Yamagishi T, Shimizu Y, Suemori H, Nakatsuji N, Mitani K. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13781-6. 10.1073/pnas.0806976105 PubMed 18768795
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