pAMHDAdGT8-4
(Plasmid
#26421)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size w/o insert (bp) 3360
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHSV-TK, mCMV-b-gal
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SpeciesE.coli, HSV
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Insert Size (bp)8240
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI, SpeI, NheI, SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAMHDAdGT8-4 was a gift from Kohnosuke Mitani (Addgene plasmid # 26421 ; http://n2t.net/addgene:26421 ; RRID:Addgene_26421)