pF154 pcDNA3.1(+)SOD1G85R
(Plasmid #26400)

• Depositing Lab: Elizabeth Fisher
• Publication: Stevens et al PLoS One. 2010 . 5(3):e9541.

Backbone

• Vector backbone: pcDNA3.1 (+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOD1
• Species: H. sapiens (human)
• Insert Size (bp): 470
• Mutation: G85R
• Entrez Gene: SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 85 of the cDNA from GGC to CGC, resulting in the G85R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5’ and 3’ end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).