pDEST_EWSR1_C2G_R650G
(Plasmid
#26382)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFRT_DESTFLAGHA
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Backbone manufacturerN/A
- Backbone size w/o insert (bp) 5299
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEWSR1
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SpeciesH. sapiens (human)
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Insert Size (bp)1971
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MutationR650G
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GenBank IDNM_005243
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Entrez GeneEWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
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Tag
/ Fusion Protein
- FLAG/HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST_EWSR1_C2G_R650G was a gift from Thomas Tuschl (Addgene plasmid # 26382 ; http://n2t.net/addgene:26382 ; RRID:Addgene_26382) -
For your References section:
RNA targets of wild-type and mutant FET family proteins. Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T. Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163. 10.1038/nsmb.2163 PubMed 22081015