Skip to main content
Addgene

pDEST8_FLAGHA_FUS_HIS
(Plasmid #26375)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDEST8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4749
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS
  • Species
    H. sapiens (human)
  • Mutation
    delta 527 (STOP codon)
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Tags / Fusion Proteins
    • FLAG/HA (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer pFASTBACF, Polyhedrin forward
  • 3′ sequencing primer pFASTBACR
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST8_FLAGHA_FUS_HIS was a gift from Thomas Tuschl (Addgene plasmid # 26375 ; http://n2t.net/addgene:26375 ; RRID:Addgene_26375)

  • For your References section:

    RNA targets of wild-type and mutant FET family proteins. Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T. Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163. 10.1038/nsmb.2163 PubMed 22081015
Related items:
Commonly requested with: