pENTR4_EWSR1_G2A_R650H
(Plasmid
#26372)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2278
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Vector typeEntry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEWSR1
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SpeciesH. sapiens (human)
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Insert Size (bp)1971
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MutationR650H
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Entrez GeneEWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pENTR-F
- 3′ sequencing primer pENTR-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR4_EWSR1_G2A_R650H was a gift from Thomas Tuschl (Addgene plasmid # 26372 ; http://n2t.net/addgene:26372 ; RRID:Addgene_26372) -
For your References section:
RNA targets of wild-type and mutant FET family proteins. Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T. Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163. 10.1038/nsmb.2163 PubMed 22081015