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pENTR4_EWSR1_C2G_R650G
(Plasmid #26371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2278
  • Vector type
    Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EWSR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1971
  • Mutation
    R650G
  • Entrez Gene
    EWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pENTR-F
  • 3′ sequencing primer pENTR-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR4_EWSR1_C2G_R650G was a gift from Thomas Tuschl (Addgene plasmid # 26371 ; http://n2t.net/addgene:26371 ; RRID:Addgene_26371)

  • For your References section:

    RNA targets of wild-type and mutant FET family proteins. Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T. Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163. 10.1038/nsmb.2163 PubMed 22081015
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