pENTR4_FLAG/HA_FUS_His6
(Plasmid
#26365)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2248
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Vector typeEntry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFUS
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SpeciesH. sapiens (human)
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Insert Size (bp)1699
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Mutationdelta 527 (stop codon)
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Entrez GeneFUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
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Tags
/ Fusion Proteins
- FLAG/HA (N terminal on insert)
- His6 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (destroyed during cloning)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer pENTR-F
- 3′ sequencing primer pENTR-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR4_FLAG/HA_FUS_His6 was a gift from Thomas Tuschl (Addgene plasmid # 26365 ; http://n2t.net/addgene:26365 ; RRID:Addgene_26365) -
For your References section:
mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma. Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, Singer S. Genes Chromosomes Cancer. 2011 May . 50(5):338-47. 10.1002/gcc.20858 PubMed 21344536