pFRT_DESTFLAGHA_eGFP
(Plasmid
#26362)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFRT_DESTFLAGHA
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Vector typeDestination Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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Insert Size (bp)719
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GenBank IDU55762
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT_DESTFLAGHA_eGFP was a gift from Thomas Tuschl (Addgene plasmid # 26362 ; http://n2t.net/addgene:26362 ; RRID:Addgene_26362) -
For your References section:
mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma. Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, Singer S. Genes Chromosomes Cancer. 2011 May . 50(5):338-47. 10.1002/gcc.20858 PubMed 21344536