pbacNeoR
(Plasmid #26349)

• Depositing Lab: Ben Lehner
• Publication: Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.

Backbone

• Vector backbone: pPD49.78
• Backbone size w/o insert (bp): 3306
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Bacterial promoter::NeoR::bacterial 3p flank
• Alt name: neo
• Species: Tn5 transposon
• Insert Size (bp): 1170

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from pPD49.78 vector (Addgene). Neomycin resistance ORF from pEGFP-C1 (Addgene). 5' and 3' sequences flanking the AmpR gene were placed upstream and downstream of NeoR to confer bacterial expression.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transform this plasmid into E. coli and use as a food source for worms grown on G418 selective plates.