pbacPuroR
(Plasmid #26348)

• Depositing Lab: Ben Lehner
• Publication: Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.

Backbone

• Vector backbone: pPD49.78
• Backbone size w/o insert (bp): 3300
• Vector type: Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Bacterial promoter::PuroR::bacterial 3p flank
• Alt name: Puromycin N-acetyl-tranferase (PAC)
• Species: Streptomyces
• Insert Size (bp): 982

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank file
• A portion of this plasmid was derived from a plasmid made by: Based on pPD49.78 vector (Addgene). Puromycin resistance gene from pBabePuro (Addgene). 5' and 3' sequences flanking the AmpR gene were placed upstream and downstream of PuroR to confer bacterial expression

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transform this plasmid into E. coli and use as a food source for worms grown on puromycin selective plates.