pBCN27-R4R3
(Plasmid #26346)

• Purpose: (Empty Backbone)
• Depositing Lab: Ben Lehner
• Publication: Semple et al Nat Methods. 2010 Aug 22. ():.

Backbone

• Vector backbone: pCJF150
• Backbone size (bp): 10809
• Vector type: Worm Expression
• Selectable markers: Puromycin ; unc-119

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB Survival T1 phage resistant cells.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AttR4 (destroyed during cloning)
• 3′ cloning site: AttR3 (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Prpl-28::PuroR::let-858_3'UTR cassette was inserted into the backbone of pCFJ150 to create a puromycin-selectable MosSCI vector for single copy integration in worms at the ttTi5605.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).