pcDNA3.2/V5 hsa-mir-499
(Plasmid #26320)

• Depositing Lab: David Bartel
• Publication: Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Backbone

• Vector backbone: pcDNA3.2/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7711
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hsa-mir-499
• Alt name: mir 499
• Alt name: mir-499
• Species: H. sapiens (human)
• Insert Size (bp): 312
• Entrez Gene: MIR499A (a.k.a. MIR499, MIRN499, hsa-mir-499a, mir-499a)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: V5 reverse (5′-ACCGAGGAGAGGGTTAGGGAT-3′ )

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.