pTomo-Ras
(Plasmid #26292)

• Depositing Lab: Inder Verma
• Publication: Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Backbone

• Vector backbone: pTomo
• Backbone manufacturer: Verma lab
• Backbone size w/o insert (bp): 8753
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HRAS-V12
• Species: H. sapiens (human)
• Insert Size (bp): 627
• Mutation: G12V
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: dsRed1-
• 3′ sequencing primer: IRES-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From the paper: To construct pTomo H-RasV12, we amplified Flag-tagged H-RasV12 from pGEM H-RasV12 by PCR and inserted it into the BamHI site of the pTomo vector.