pTomo
(Plasmid #26291)

• Purpose: (Empty Backbone)
• Depositing Lab: Inder Verma
• Publication: Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Backbone

• Vector backbone: p156RRLsin PPTCMVIRESPRE
• Backbone manufacturer: Naldini lab
• Backbone size (bp): 8753
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: empty

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate pTomo, the inserted loxP-mRFP-loxP fragment amplified from the pSETB mRFP1 vector by PCR between the XbaI and BamHI sites of the p156RRLsin PPTCMVIRESPRE vector (a third-generation lentiviral vector).

Note that the Addgene quality control sequence detected a G->A mutation in the IRES. It is not known if or how this mutation affects function.