pLenti CMV/TO GFP-MDC1 (779-2)
(Plasmid #26285)

• Depositing Lab: Eric Campeau
• Publication: New vectors Aug. 2010 (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 7796
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mediator of DNA damage checkpoint 1
• Alt name: MDC1
• Alt name: NFBD1
• Alt name: KIAA0170
• Species: H. sapiens (human)
• Insert Size (bp): 6269
• Entrez Gene: MDC1 (a.k.a. NFBD1)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bst BI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMVforw
• 3′ sequencing primer: WPRErev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MDC1 cDNA was derived from the clone KIAA0170 (Kazusa).
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Functionality of the GFP-MDC1 fusion protein was verified by co-localization with gamma-H2AX after DNA damage.