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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26284 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3270
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMediator of DNA damage checkpoint 1
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Alt nameMDC1
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Alt nameNFBD1
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Alt nameKIAA0170
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SpeciesH. sapiens (human)
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Insert Size (bp)6269
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Entrez GeneMDC1 (a.k.a. NFBD1)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bst BI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMDC1 cDNA was derived from clone KIAA0170 (Kazusa)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The functionality of the GFP-MDC1 protein was verified by co-localization with gamma-H2AX after DNA damage.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-GFP-MDC1 (770-7) was a gift from Eric Campeau (Addgene plasmid # 26284 ; http://n2t.net/addgene:26284 ; RRID:Addgene_26284)
Map uploaded by the depositor.
