pENTR4-GLU-GLU (w719-1)
(Plasmid
#26283)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR4
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Backbone manufacturerInvitrogen
- Backbone size (bp) 2324
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameno insert
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Tag
/ Fusion Protein
- GLU-GLU (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xmn I (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GLU-GLU epitope tag for N-terminal fusions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR4-GLU-GLU (w719-1) was a gift from Eric Campeau (Addgene plasmid # 26283 ; http://n2t.net/addgene:26283 ; RRID:Addgene_26283)