pMSP2
(Plasmid #26282)

• Depositing Lab: Stephen Sligar
• Publication: Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Backbone

• Vector backbone: pET 28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MSP2
• Alt name: membrane scaffold protein;
• Alt name: apolipoprotein A-1
• Alt name: APOA1
• Species: synthetic gene
• Insert Size (bp): 1280
• Mutation: synthetic gene of dimer of deletion mutant (1-43) of human Apolipoprotein A-1; contains N-terminal 6-His tag and Factor X site; the codons optimized for E. coli expression
• Tag / Fusion Protein:
  • 6-His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

BL-21(DE3) for expression; high oxygenation level.

First described in the following paper: Bayburt, T. H., Grinkova, Y. V., & Sligar, S. G., 2002, Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nanoletters, 2: 853-856.