pGL2- Empty control -SV40-Luc
(Plasmid #26280)

• Purpose: (Empty Backbone) Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.
• Depositing Lab: Rudolf Jaenisch
• Publication: Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.

Backbone

• Vector backbone: pGL2-SV40-Luc
• Backbone size (bp): 6000
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pGL2-Control plasmid

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone kindly provided by Dr. Ron Mckay and Dr.Paul Tesar (Tesar et al. Nature 2007)