SPRIZ_loops_mutant_AAV_TBG
(Plasmid
#262591)
-
PurposeAAV transfer vector for liver-specific expression of the SPRIZ loops mutant from the TBG promoter.
-
Depositing Lab
-
Depositing OrganizationUniversity of Ottawa
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262591 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepAAV.TBG.PI.Null.bGH
-
Backbone manufacturerJames M. Wilson and Penn Vector Core, Addgene plasmid #105536
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCcdc127 loops mutant
-
Alt nameSPRIZ loops mutant
-
Alt nameCCDC127
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)780
-
MutationL176D; I177D; I178D; V222D; W223D
-
GenBank IDNM_001168659.1
-
Entrez GeneCcdc127 (a.k.a. AI256405, AW050060, A430066A18, C130002N06, 0610011N22Rik, 5430420E17Rik)
- Promoter TBG
Cloning Information
- Cloning method Unknown
Resource Information
-
A portion of this plasmid was derived from a plasmid made bySynthetic derivative of Mus musculus Ccdc127; RefSeq NM_001168659.1
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutations were determined directly from the supplied sequence. Backbone/growth reference: https://www.addgene.org/105536/ ; gene reference: https://www.ncbi.nlm.nih.gov/gene/67433/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SPRIZ_loops_mutant_AAV_TBG was a gift from Luca Pellegrini (Addgene plasmid # 262591)