MARTINI_AAV_TBG
(Plasmid #262590)

• Purpose: AAV transfer vector for liver-specific expression of MARTINI, a lengthened SPRIZ variant, from the TBG promoter.
• Depositing Lab: Luca Pellegrini
• Depositing Organization: University of Ottawa
  Located in Canada
• Publication: SPRIZ (CCDC127) protein conserved in deuterostome animals is a rigid OMM-IMM tether that locally sets mitochondrial IMS volume (unpublished)

Backbone

• Vector backbone: pAAV.TBG.PI.Null.bGH
• Backbone manufacturer: James M. Wilson and Penn Vector Core, Addgene plasmid #105536
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MARTINI
• Alt name: SPRIZ lengthened variant
• Alt name: CCDC127
• Species: M. musculus (mouse)
• Insert Size (bp): 1008
• Mutation: Internal 75-aa tandem duplication of SPRIZ residues 58-132
• GenBank ID: NM_001168659.1
• Entrez Gene: Ccdc127 (a.k.a. AI256405, AW050060, A430066A18, C130002N06, 0610011N22Rik, 5430420E17Rik)
• Promoter: TBG

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthetic derivative of Mus musculus Ccdc127; RefSeq NM_001168659.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The supplied sequence contains a codon-optimized duplicated region. Backbone/growth reference: https://www.addgene.org/105536/ ; gene reference: https://www.ncbi.nlm.nih.gov/gene/67433/