pZE31-DsRed-ms2x2
(Plasmid #26248)

• Depositing Lab: Philippe Cluzel
• Publication: Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73.

Backbone

• Vector backbone: pZE31-DsRed
• Backbone manufacturer: constructed in Cluzel Lab
• Backbone size w/o insert (bp): 2600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ms2 binding sites (x2)
• Insert Size (bp): 200

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: PLtetO-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The tandem ms2 binding sites were cloned from the pIIIMS2-2 plasmid, which was a gift from K Bloom and UNC Chapel Hill. DsRedT3f was cloned from pQE31-DsRed.T3f from Dan Strongnin and the Glick Lab, U Chicago The vector was a gift from H. Bujard to C. Guet.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry