pZS*12-MS2-GFP
(Plasmid #26245)

• Depositing Lab: Philippe Cluzel
• Publication: Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73.

Backbone

• Vector backbone: pZS*12
• Backbone manufacturer: Lutz and Bujard, Nucleic Acids Research 25 (1997) 1203-1210.
• Backbone size w/o insert (bp): 3659
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MS2-GFP
• Alt name: MS2 coat protein and GFP fusion
• Species: fusion
• Insert Size (bp): 1083
• Mutation: five amino acid linker (Ser, Gly, Gly, Gly, Gly) between the C terminus of the dlFG mutant MS2 coat protein and the N terminus of GFP. The MS2 coat protein is a dlFG mutant with a deletion for amino acids 68-82.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: PLlacO-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MS2-GFP fusion constructed in Le et al PNAS 2005 (also from Cluzel lab). pZS*12 vector was a gift from H. Bujard to C. Guet.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry