pcDNA3 IKKE K38A flag
(Plasmid #26202)

• Depositing Lab: Tom Maniatis
• Publication: Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: I-kappa-B kinase epsilon K38A
• Alt name: ikbke
• Alt name: IKKe
• Alt name: IKKi
• Species: H. sapiens (human)
• Insert Size (bp): 2154
• Mutation: Catalytic Lysine changed to Alanine (K38A)
• GenBank ID: NM_014002
• Entrez Gene: IKBKE (a.k.a. IKK-E, IKK-i, IKKE, IKKI)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry