pAAV-EF1a-FLEX-GT
(Plasmid #26198)

• Depositing Lab: Edward Callaway
• Publication: Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21848-53. doi: 10.1073/pnas.1011756107

Backbone

• Vector backbone: pAAV-double floxed-EYFP-WPRE-pA
• Backbone manufacturer: Karl Deisseroth lab
• Backbone size w/o insert (bp): 5300
• Vector type: AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Invitrogen One Shot Stbl3 chemically competent cells
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Enhanced green fluorescent protein, TVA
• Alt name: tv-a
• Alt name: GFP
• Alt name: eGFP
• Species: M. musculus (mouse); TVA is a mouse codon optimized version of an avian gene.
• Insert Size (bp): 1600
• Mutation: TVA mammalian codon optimized
• Entrez Gene: eGFP (a.k.a. pPRS3a_01)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EF1a_fwd_primer

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry