XMRV vp62
(Plasmid #26189)

• Depositing Lab: Joseph DeRisi
• Publication: Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XMRV VP62
• Alt name: Xenotropic MuLV-related virus VP62
• Species: H. sapiens (human)
• Insert Size (bp): 8165
• GenBank ID: EF185282
• Entrez Gene: gag (a.k.a. XMVV62_gp3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Dr. Robert Silverman in the Department of Cancer Biology, Lerne Research Institute and Glickman Urologic Institute, Clevl=eland Clinic, 9500 Eucid Avenue, Cleveland OH 44195
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry