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Addgene

pMLLKA-J72039
(Plasmid #26184)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26184 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMLL-KA
  • Backbone size w/o insert (bp) 2852
  • Vector type
    Synthetic Biology ; 2ab assembly vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Other
  • Growth instructions
    Provided in a pir+ strain. Grow at 37C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Med7 aa102-205 for C-terminal tagging
  • Alt name
    Bmll3
  • Alt name
    cMED7
  • Alt name
    J72039
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    356

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gtatcacgaggcagaatttcag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that this plasmid needs to be grown in both Kanamycin and Ampicillin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMLLKA-J72039 was a gift from Christopher Anderson (Addgene plasmid # 26184 ; http://n2t.net/addgene:26184 ; RRID:Addgene_26184)
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