Skip to main content
Addgene

CD11b promoter in pGEM3zf(-)
(Plasmid #26168)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26168 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEM3zf-
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3199
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD11b promoter
  • Alt name
    D604
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1787
  • GenBank ID
    M84477 M76724 M80772
  • Entrez Gene
    ITGAM (a.k.a. CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The cloned CD11b promoter in this construct contains some 5'UTR, including the transcription start site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD11b promoter in pGEM3zf(-) was a gift from Daniel Tenen (Addgene plasmid # 26168 ; http://n2t.net/addgene:26168 ; RRID:Addgene_26168)

  • For your References section:

    The CD11b promoter directs high-level expression of reporter genes in macrophages in transgenic mice. Dziennis S, Van Etten RA, Pahl HL, Morris DL, Rothstein TL, Blosch CM, Perlmutter RM, Tenen DG. Blood. 1995 Jan 15. 85(2):319-29. PubMed 7811988
Related items:
Commonly requested with: