CMV-d2eGFP-empty
(Plasmid #26164)

• Depositing Lab: Phil Sharp
• Publication: Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Backbone

• Vector backbone: modified pcDNA5
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Insert Size (bp): 5000
• Mutation: …ccgcCTCGAG[sponge sites]GGGCCCgttt… became …ccgcCTCGACgttt…

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: ApaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is to be used ONLY as a negative control for the CMV-d2eGFP plasmids in the associated paper. The multiple cloning site has been destroyed and should not be used for further cloning.