pMV306hsp+FFluc
(Plasmid #26156)

• Depositing Labs: Brian Robertson, Siouxsie Wiles
• Publication: Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Backbone

• Vector backbone: pMV306hsp
• Backbone size w/o insert (bp): 4373
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Firefly luciferase
• Alt name: FFluc
• Alt name: Luc
• Species: Photinus pyralis
• Insert Size (bp): 1691
• Mutation: Firefly luciferase gene codon optimised for mycobacteria. Lacks the last 3aa

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: agaataacgttggcactcgc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mycobacteria optimized firefly luciferase gene cloned from pJ246:17659 obtained from Jeff Cirillo (Texas A&M) and Kevin Francis (Caliper Life Sciences)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry