pCR2.1-eIF3c
(Plasmid
#26153)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3900
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Vector typecloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmed-eIF3c
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Alt nameeIF3c
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SpeciesSchmidtea mediterranea
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Insert Size (bp)1539
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR2.1-eIF3c was a gift from Phillip Newmark (Addgene plasmid # 26153 ; http://n2t.net/addgene:26153 ; RRID:Addgene_26153) -
For your References section:
A functional genomic screen in planarians identifies novel regulators of germ cell development. Wang Y, Stary JM, Wilhelm JE, Newmark PA. Genes Dev. 2010 Sep 15. 24(18):2081-92. 10.1101/gad.1951010 PubMed 20844018