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Addgene

pPR244-sped-1
(Plasmid #26151)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPR244
  • Backbone size w/o insert (bp) 2650
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    HT115(DE3)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Smed-sped-1
  • Alt name
    sped-1
  • Species
    Schmidtea mediterranea
  • Insert Size (bp)
    840

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CCTCGAGGTCGACGGTATC
  • 3′ sequencing primer AACAAAAGCTGGAGCTCCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPR244-sped-1 was a gift from Phillip Newmark (Addgene plasmid # 26151 ; http://n2t.net/addgene:26151 ; RRID:Addgene_26151)

  • For your References section:

    A functional genomic screen in planarians identifies novel regulators of germ cell development. Wang Y, Stary JM, Wilhelm JE, Newmark PA. Genes Dev. 2010 Sep 15. 24(18):2081-92. 10.1101/gad.1951010 PubMed 20844018
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