I-SceI pB 5UAS::ZfSyGCaMP2
(Plasmid #26125)

• Depositing Lab: Leon Lagnado
• Publication: Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Backbone

• Vector backbone: pBluescript II (KS)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3585
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synaptophysin GCaMP2
• Alt name: SyGCaMP2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2262
• Entrez Gene: sypb (a.k.a. zgc:136469, zgc:92837)
• Tag / Fusion Protein:
  • GCaMP2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: M13-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Reference the following (for GCaMP2) when using this plasmid, along with the original article: Tallini YN, Ohkura M, Choi B-R, Ji J, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin H-B, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high signal Ca2+ indicator G-CaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

At both ends of the multiple cloning site of the original pBluescript II KS+, a I-SceI recognition site was cloned replacing the BssHII cutting sequence.

In the map, ORF frame 3 (1107-3368): zebrafish SyGCaMP2. Also, 5UAS is located 668-1034.