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Addgene

pXL001
(Plasmid #26122)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSin-EF2-Nanog
  • Backbone size w/o insert (bp) 7569
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3 37C 18hours
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tTR-KRAB
  • Alt name
    tTA-KRAB
  • Species
    trans-activator - KRAB
  • Insert Size (bp)
    1008
  • Tag / Fusion Protein
    • IRES-Puro (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer cPPT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXL001 was a gift from Sean Palecek (Addgene plasmid # 26122 ; http://n2t.net/addgene:26122 ; RRID:Addgene_26122)

  • For your References section:

    Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK, Zhang J, Kamp TJ, Palecek SP. Proc Natl Acad Sci U S A. 2012 May 29. 10.1073/pnas.1200250109 PubMed 22645348
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