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Addgene

piiTRE-Bi-SG-Tii
(Plasmid #26121)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBi
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Synaptophysin
  • Alt name
    Syp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    923
  • Entrez Gene
    Syp (a.k.a. A230093K24Rik, Syn, p38)
  • Tag / Fusion Protein
    • mut4EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are 6 mismatches between author sequence and Addgene sequence but they do not alter the amino acid sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piiTRE-Bi-SG-Tii was a gift from Liqun Luo (Addgene plasmid # 26121 ; http://n2t.net/addgene:26121 ; RRID:Addgene_26121)

  • For your References section:

    Visualizing the distribution of synapses from individual neurons in the mouse brain. Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L. PLoS One. 2010 . 5(7):e11503. 10.1371/journal.pone.0011503 PubMed 20634890
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