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Addgene

pRK-ATF4 111-351
(Plasmid #26119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-A20 (TNFAIP3)
  • Backbone manufacturer
    Dr. Hongbin Shu
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    720
  • Mutation
    aa's 1-110 have been deleted
  • Entrez Gene
    ATF4 (a.k.a. CREB-2, CREB2, TAXREB67, TXREB)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK-ATF4 111-351 was a gift from Yihong Ye (Addgene plasmid # 26119 ; http://n2t.net/addgene:26119 ; RRID:Addgene_26119)

  • For your References section:

    ERAD inhibitors integrate ER stress with an epigenetic mechanism to activate BH3-only protein NOXA in cancer cells. Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, Ron D, Chen W, Trenkle W, Wiestner A, Ye Y. Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5. 10.1073/pnas.0807611106 PubMed 19164757
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