pRK-ATF4
(Plasmid #26114)

• Depositing Lab: Yihong Ye
• Publication: Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.

Backbone

• Vector backbone: pRK5-A20 (TNFAIP3)
• Backbone manufacturer: Dr. Hongbin Shu
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATF4
• Species: H. sapiens (human)
• Insert Size (bp): 1050
• Entrez Gene: ATF4 (a.k.a. CREB-2, CREB2, TAXREB67, TXREB)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry