pMBP_hBrg1 (J1 antibody)
(Plasmid
#26109)
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Depositing Lab
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Publication
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMAL-c2X
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 6646
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBrg1
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SpeciesH. sapiens (human)
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Insert Size (bp)671
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Entrez GeneSMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
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Tag
/ Fusion Protein
- MBP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Hind3 (not destroyed)
- 5′ sequencing primer TCGAGCTCGAACAACAAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBP_hBrg1 (J1 antibody) was a gift from Jerry Crabtree (Addgene plasmid # 26109 ; http://n2t.net/addgene:26109 ; RRID:Addgene_26109) -
For your References section:
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex. Wang W, Cote J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, Workman JL, Crabtree GR. EMBO J. 1996 Oct 1. 15(19):5370-82. PubMed 8895581
